Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 11;10(8):147. doi: 10.3390/life10080147

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

FRET analysis of ubiquitous inter-AFB expressed in i36 cells is not efficient in detecting αS aggregation under normal or pro-aggregating conditions. Induced i36 cells were transfected and assayed following the same protocol as described in Figure 3. (A) Images of AP FRET efficiency showed high FRET values (red regions) for the Ctrl + and a distribution of lower FRET values (blue and green regions) for all the other conditions comparable to the Ctrl–Scale bar, 10 μm. (B) Box plot of FRET efficiency indicated that only samples treated with leupeptin or microsome-associated αS aggregates show significant values compared to Ctrl–but not when compared to inter-AFB in untreated condition. *** (p < 0.001); **** (p < 0.0001); n.s., not significant. (C) WB analysis of Tx-S fraction of cell lysates transfected with inter-AFB in untreated conditions or treated with pro-aggregating agents. Immunoblotting analysis with Syn-1 or GFP antibody of Tx-S fractions of i36 cell lines transfected with inter-AFB shows the correct expression of the biosensor with the predicted molecular weight of about 45kDa in treated and control conditions.