Figure 5.
Impact of CBCA treatment on B. subtilis cell wall integrity. B. subtilis cells in exponential phase (OD600 0.1–0.3) were not treated (A,C,E,G) or treated with CBCA at 0.4 × MIC (B,D,F,H) as well as the lipid-staining fluorescent molecule AM466 (C–F). After 30 min, DAPI (200 ng/mL) was added, cell suspensions deposited on agarose pads for fluorescence or phase contrast microscopy. White arrows indicate long-spindle shaped nucleoids and smearing of nuclear material (row 1); abrogation of the lipid membrane (row 2); maintenance of rod-shape of Bacillus simultaneous to degradation of nucleoids and lipid membrane (row 4).