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. 2020 Aug 15;18(8):428. doi: 10.3390/md18080428

Figure 6.

Figure 6

Actinomycin induces increased reactive oxygen species (ROS) levels. Flow cytometry was used to obtain the peak figure of ROS level after actinomycin (0–8 nM) was added to cells and incubated for 24 h: (A) Act V in LO-2; (B) Act V in 293T; (C) Act D in LO-2; (D) Act D in 293T; (E) ROS increase induced by actinomycin in LO-2 cells compared with the control group; (F) ROS increase induced by actinomycin in 293T cells compared with the control group. Experiments were repeated three times: * p < 0.05; *** p < 0.001 vs. Act V 0 nM; # p < 0.05; ## p < 0.01 vs. Act D 0 nM; △ p < 0.05; △△ p < 0.01 vs. Act D.