ACT	actinorhodin
AGOS	artificial gene operon assembly system
AICE	actinomycetes integrative and conjugative elements
aMSGE	advanced multiplexed site-specific genome engineering
BAC	bacterial artificial chromosome
BGC	biosynthetic gene cluster
CATCH	Cas9-assisted targeting of chromosome segments
CRISPR/Cas	clustered regularly interspaced short palindromic repeats/CRISPR associated
crRNA	CRISPR RNA
DiPaC	Direct pathway cloning
DSB	double-strand break
DNA	deoxyribonucleic acid
gDNA	genomic DNA
GFP	green fluorescent protein
GusA	glucuronidase
HDR	homology directed repair
HE	homing endonuclease
MCS	multiple cloning site
mRNA	messenger RNA
MSGE	multiplexed site-specific genome engineering
NHEJ	non-homologous end joining
NRPS	nonribosomal peptide synthetase
OTC	oxytetracycline
PCR	polymerase chain reaction
PKS	polyketide synthase
PTM	polycyclic tetramate macrolactam
qPCR	quantitative PCR
RBS	ribosome binding sites
Real-time RT-PCR	real-time reverse-transcription quantitative polymerase
RNA	ribonucleic acid
SD	Shine-Dalgarno
SEVA	standard European vector architecture
sgRNA	synthetic guide RNA
SLIC	sequence- and ligation-independent cloning
TAR	transformation associated recombination
tracrRNA	trans-activating CRISPR RNA
UTR	untranslated region
YAC	yeast artificial chromosome