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. 2020 Aug 5;21(16):5605. doi: 10.3390/ijms21165605

Figure 1.

Figure 1

Characterization of Morrbid long noncoding RNA (lncRNA) expression, cellular localization and knockdown phenotype. (A) Comparison of Morrbid expression levels in AML12 normal murine hepatocytes and the Hepa1-6 hepatoma cell line with RT-qPCR. (B) Fluorescence in situ hybridization analysis (FISH) of Morrbid localization in AML12 cells (DNA was stained with Dapi and Morrbid was stained with a Cy5-labeled probe). (C) RT-qPCR analysis of Morrbid expression in the nuclear and cytoplasmic fractions extracted from AML12 cells. (D) Viability assay of Hepa1-6 and AML12 cells depleted in Morrbid, on the 1, 1.5, 2, 3 and 4 days of knockdown, normalized to control the luciferase antisense oligonucleotides (LUC ASO) treatment and viability at day 1 after the initial transfection. (E) Wound-healing assay of Morrbid knockdown (KD) and LUC control KD in AML12 and Hepa1-6 cells. The wound was introduced just after the initial transfection, and data were normalized to the wound area at the first timepoint. Results show mean ± SD. n.s.—not significant. * p < 0.05, ** p < 0.01 and *** p < 0.001.