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. 2020 Aug 5;21(16):5605. doi: 10.3390/ijms21165605

Figure 2.

Figure 2

Morrbid lncRNA depletion leads to the enhanced incorporation of the NRAS premature termination codons (PTC) exon in murine hepatocytes. (A) RNA coverage of the zoomed region of the NRAS transcript in Morrbid and LUC control knockdowns. The solid and dashed arced lines represent the RNA coverage of the splice junctions. (B) RT-qPCR analysis of the NRAS isoform expression after Morrbid KD and control LUC KD using primers laying on the junction between the PTC exon and neighboring exons (PTC junction) and spanning across the junction to the downstream neighboring exon (PTC down). (C) Image of amplicon separation by agarose gel electrophoresis. Amplicons were obtained with primers spanning across the alternative NRAS exon to amplify the PTC and no PTC NRAS transcripts. (D) RT-qPCR analysis of the NRAS pre-mRNA level in the control LUC and Morrbid KD cells using primers that amplify the fragment with exon1 and intron. NMD: nonsense-mediated decay. Results show mean ± SD. n.s.—not significant. * p < 0.05, ** p < 0.01 and *** p < 0.001.