Figure 3.

The abundance of mRNA expressed for markers involved in adiponectin signalling, fatty acid oxidation and oxidative capacity in C₂C₁₂ myotubes treated for 24 h with O-1602 (10–1000 nM). mRNA expression was normalised to housekeeping gene Hypoxanthine Phosphoribosyltransferase (HPRT1) and grouped data is reported as mean (arbitrary units) ± SEM. (a) Nuclear Factor of Activated T-Cells calcineurin dependent 1 (NFATc1); (b) Peroxisome proliferator-activated receptor gamma co activator 1-alpha (PGC1α); (c) Adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and leucine zipper motif 1 (APPL1); (d) Adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and leucine zipper motif 2 (APPL2).