Table 2.
Forward and Reverse Oligonucleotide Primer Sequences for ‘Real Time’ Polymerase Chain Reaction.
| Primer | Accession Number | Direction | Sequence |
|---|---|---|---|
| Rat Genes | |||
| Cyclophilin | NM_017101.1 | Forward (5′ 3′) | CTG ATG GCG AGC CCT TG |
| Reverse (5′ 3′) | TCT GCT GTC TTT GGA ACT TTG TC | ||
| β-Actin | NM_031144 | Forward (5′ 3′) | CTA AGG CCA ACC GTG AAA TGA |
| Reverse (5′ 3′) | CCA GAG GCA TAC AGG GAC AAC | ||
| GPR18 | NM_001079710.1 | Forward (5′ 3′) | GTG GGG GTC TGG ATA ATG AC |
| Reverse (5′ 3′) | CGC GTG AAG TTA AGC ACA TT | ||
| AdipoR1 | NM_207587.1 | Forward (5′ 3′) | TGA GGT ACC AGC CAG ATG TC |
| Reverse (5′ 3′) | CGT GTC CGC TTC TCT GTT AC | ||
| APPL1 | XM_008771023.1 | Forward (5′ 3′) | TCA CTC CTT CCC CAT CTT TC |
| Reverse (5′ 3′) | TAG AGA GAG GGC AGC CAA AT | ||
| APPL2 | NM_001108741.1 | Forward (5′ 3′) | TGC TCG GGC TAT TCA CAA |
| Reverse (5′ 3′) | AAA CAG GCC CGT GAC ACT | ||
| PGC1α | NM_031347.1 | Forward (5′ 3′) | ACC CAC AGG ATC AGA ACA AACC |
| Reverse (5′ 3′) | GAC AAA TGC TCT TTG CTT TAT TGC | ||
| FOXO1 | NM_001191846.2 | Forward (5′ 3′) | CTC GGC GGG CTG GAA |
| Reverse (5′ 3′) | TCA TTC TGT ACT CGA ATA AAC TTG | ||
| PDK4 | NM_053551.1 | Forward (5′ 3′) | GGG ATC TCG CCT GGC ACT TT |
| Reverse (5′ 3′) | CAC ACA TTC ACG AAG CAG CA | ||
| βHAD | AF095449.1 | Forward (5′ 3′) | TCG TGA CCA GGC AAT TCG T |
| Reverse (5′ 3′) | CCG ATG ACC GTC ACA TGC T | ||
| FAT/CD 36 | NM_031561.2 | Forward (5′ 3′) | GAC CAT CGG CGA TGA GAA A |
| Reverse (5′ 3′) | CCA GGC CCA GGA GCT TTA TT | ||
| Mouse Genes | |||
| HPRT1 | NM_013556.2 | Forward (5′ 3′) | GCAAACTTTGCTTTCCCTGG |
| Reverse (5′ 3′) | ACTTCGAGAGGTCCTTTTCAC | ||
| NFATc1 | NM_016791.3 | Forward (5′ 3′) | TCCAAAGTCATTTTCGTGGA |
| Reverse (5′ 3′) | GTTGCGGAAAGGTGGTATCT | ||
| PGC1α | NM_008904.1 | Forward (5′ 3′) | CACCCACAGGATCAGAACAA |
| Reverse (5′ 3′) | GGTCATCGTTTGTGGTCAGA | ||
| APPL1 | NM_145221.2 | Forward (5′ 3′) | ATCAGGCGGAAGAAGTGAGA |
| Reverse (5′ 3′) | TTTCTGATGCCCTACGATCC | ||
| APPL2 | NM_145220.2 | Forward (5′ 3′) | CCAAAAGTATGGACGGCTTC |
| Reverse (5′ 3′) | CTCAGCTTCCAGTTCCACCT | ||
| AMPKα2 | NM_178143.1 | Forward (5′ 3′) | GCCCAGATGAACGCTAAGAT |
| Reverse (5′ 3′) | TGCATACAGCCTTCCTGAGA | ||
| PDK4 | NM_013743.2 | Forward (5′ 3′) | GAGAAGAGCCCAGAAGACCA |
| Reverse (5′ 3′) | TCCACTGTGCAGGTGTCTTT | ||
| Human Genes | |||
| Cyclophilin | NM 021130.3 | Forward (5′ 3′) | CATCTGCACTGGCAAGACTGA |
| Reverse (5′ 3′) | TTCATGCCTTCTTTCACTTTGC | ||
| NFATc1 | NM_172390.1 | Forward (5′ 3′) | CCT CTC CAA CAC CAA AGTCC |
| Reverse (5′ 3′) | CGA TGT CCG TCT CTC CTT TC | ||
| PGC1α | NM_013261 | Forward (5′ 3′) | CAAGCCAAACCAACAACTTTATCTCT |
| Reverse (5′ 3′) | CACACTTAAGGTGCGTTCAATAGTC | ||
| AdipoR1 | NM_015999 | Forward (5′ 3′) | CGCCATGGAGAAGATGGAA |
| Reverse (5′ 3′) | TCATATGGGATGACCCTCC | ||
| APPL1 | NM_012096 | Forward (5′ 3′) | TCACTCCTTCCCCATCTTTC |
| Reverse (5′ 3′) | TAGAGAGAGGGCAGCCAAAT | ||
| APPL2 | NM_018171 | Forward (5′ 3′) | CACGCCCAATGGAAAATC |
| Reverse (5′ 3′) | CGACTGCCTCAGGGTTGT |
AdipoR1; Adiponectin Receptor 1, AMPKα2; 5′adenosine monophosphate-activated protein kinase α-2, APPL1; Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1, APPL2; Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2, βActin; beta actin, βHAD; beta-hydroxyacyl-CoA dehydrogenase, FAT/CD36; Fatty Acid Translocase/ Cluster of Differentiation 36, FOXO1; Forkhead box protein O1, GPR18; G Protein-Coupled Receptor 18, HPRT1; Hypoxanthine-Guanine Phosphoribosyltransferase, NFATc1; nuclear factor of activated T-cells c1, PDK4; Pyruvate Dehydrogenase Kinase 4, PGC1α; Peroxisome proliferator-activated receptor gamma co-activator 1 alpha.