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. 2020 Aug 5;21(16):5618. doi: 10.3390/ijms21165618

Figure 4.

Figure 4

VPg88–111 propensity to fold into an α-helical conformation. (A) Far UV-CD spectra of VPg88–111* in the absence (dashed line), or in the presence of 10% (dotted line) and 20% (solid line) of TFE. Inset, AEDANS to fluorescein RET increase upon TFE addition (mean of three to six measurements). (B) Steady state titration of eIF4E binding to VPg88–111*. The solution contained 250 nM of VPg88–111*. AEDANS was excited at 337 nm. The solid line represents the best fit of the simple bimolecular equilibrium model to the experimental data.