| LAB | lactic acid bacteria |
| CRISPRi | CRISPR interference |
| sgRNA | single guide RNA |
| SpdCas9 | catalytically deactivated Cas9 of Streptococcus pyogenes |
| BCD | bicistronic design |
| RBS | ribosome binding sites |
| sfGFP | superfolder green fluorescent protein |
| PAM | protospacer adjacent motif |
| qRT-PCR | quantitative reverse transcription PCR |
| HPLC | high performance liquid chromatography |
| PBS | phosphate-buffered saline |
| TBS-T | Tris-buffered saline with Tween-20 solution |
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