Skip to main content
. 2020 Aug 17;21(16):5909. doi: 10.3390/ijms21165909

Figure 3.

Figure 3

Inhibition of PMTPV-induced decrease in CLDN1 expression by endocytosis and lysosome inhibitors. A549 cells were incubated with PMTPV at 100 μM for 24 h in the presence and absence (vehicle) of 10 μM chloroquine (CQ) or 5 μM monodansylcadaverine (MDC). (A) The cell lysates were immunoblotted with anti-CLDN1 and anti-β-actin antibodies. The protein level of CLDN1 is represented relative to the values in vehicle. (B) The cells were stained with anti-CLDN1 (red), anti-ZO-1 (green) antibodies. Merged images with 4′,6-diamidino-2-phenylindole (DAPI) (blue) are indicated on the right. Scale bar represents 10 µm. n = 3–4. * p < 0.05 compared with vehicle. # p < 0.05 compared with PMTPV alone.