FIGURE 2.

Stimulation with viruses and interferons upregulate cGAS but not STING expression in pDCs (A, B) Purified pDCs were stimulated with HSV, SeV, IAV, HIV, IFN-α, or IFN-λ for 4 hours. Total RNA was collected and cDNA was prepared. qPCR was performed to test how the expression of cGAS and STING is altered following each stimulation and are expressed relative to mock (n = 5 independent experiments). (C, D) PBMCs were incubated with IFN-α, IFN-λ, HSV, SeV, IAV, and HIV for 8 hours and expression of (C) cGAS and (D) STING in pDC (CD123⁺/BDCA2⁺ cells) were measured by flow cytometry and data expressed as the median fluorescent intensity (MFI) for the mock vs. stimulated pairs, with each donor represented by a line (n=5 independent experiments). Pairwise T tests were performed to test both mRNA and protein expression affected by each stimulation as compared to Mock.