Skip to main content
. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: J Immunol. 2020 May 29;205(1):223–236. doi: 10.4049/jimmunol.1800933

FIGURE 7.

FIGURE 7.

Pre-stimulation of cGAS-STING pathway inhibits TLR9-mediated cytokine production in pDCs probably by inducing SOCS1/3 but not SOCS5. (A) pDCs were stimulated for 6 hours for cGAS-STING pathway (via cGAMP or ISD) or TLR9 pathway (via CpG-A) followed by stimulation with HSV or CpG-A for 18 hours. Supernatants were collected and ELISAs were performed to measure IFN-α (n =3 independent experiments). (B) pDCs stimulated with cGAMP or ISD for 3 hours and qRT-PCR was performed to measure the induction of SOCS1, SOCS3, and SOCS5. (n =3 independent experiments) (C) pDCs pre-treated with or without anti IFNAR antibody for 1 hour were stimulated with cGAMP, ISD, or IFN-α for 3 hours. qRT-PCR was performed to measure the induction of SOCS1 and SOCS3 (n = 3 independent experiments).