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. 2020 Sep 1;11:89. doi: 10.1186/s40104-020-00497-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Metabolic activity of murine macrophages after treatment with components of Saccharomyces cerevisiae. Metabolic activity was assessed by metabolism of resazurin to the fluorescent product resorufin. Untreated RAW cells represent the negative control, and RAW cells stimulated with 0.1 μg/mL LPS served as the positive control. Increasing doses (0.01, 0.1, or 1 mg/mL) are signified by increasing color intensity within treatment compound. Solid bars indicate control or cells treated with yeast components, whereas patterned bars indicate cells stimulated with LPS or both LPS and yeast components. Values are reported as LSM ± SE. * indicates means (yeast components alone) differ significantly from CON, # indicates means (yeast components following LPS) differ significantly from LPS (P < 0.05, Tukey’s HSD). Different letters indicate means differ (P < 0.05, Tukey’s HSD). Overall, yeast treatment interacted with LPS stimulation (interaction, P < 0.01) on cellular metabolism of resazurin