Fig. 7.

Histone H3K4me3 signal intensities are associated with active gene expression. a TPM distribution of PCGs. b Box plot showing normalized transcript levels (log₂TPM), determined by mRNA-seq, at two subclasses of genes that with or without H3K4me3 peaks in CUT&Tag-seq or ChIP-seq. Mean values of A and B indicate significant different between two subclasses (P < 0.001, Fisher Pairwise Comparisons). c Box plot showing normalized H3K4me3 peak signals (log₂TPM), determined by CUT&Tag-seq, at a number of different subclasses of genes that ranged and artificially divided by TPM values from mRNA-seq. Blue box: Normalized read counts of PCGs from mRNA-seq by log₂TPM. Red box: Normalized read counts of peaks from CUT&Tag-seq by log₂CPM. Mean values of log₂CPM of the H3K4me3 peak signals were indicated. Values that do not shared a letter (from A to F) are significant different (P < 0.001, Fisher Pairwise Comparisons)