Fig. 2.

RNAseq-based transcriptomic analyses after 72-h heme treatment in Aag2 cells in Schneider’s Drosophila medium. Aag2 cells grown with FBS, without FBS (0 μM heme) or without FBS+ 10 μM heme (a-c) Log2 fold change (LogFC) vs Log10 counts per million (LogCPM) graph of genes found differentially expressed in analysis for each comparison. Genes with an adjusted p-value corrected for multiple testing (False discovery rate: FDR) of < 0.0001 are shown in red. d-f Volcano plot of genes found differentially expressed. a&d FBS vs 0 μM heme (b&e) FBS vs 10 μM Heme (c&f) 0 μM heme vs 10 μM heme. g 15 gene expression profile clusters were identified through soft clustering. Only genes with membership scores > 50% are shown in each cluster. Red lines indicate high membership of a gene in a particular cluster, while blue indicates low membership. Heme import-like clusters are highlighted in red and export-like clusters in blue. The different heme treatment groups are given on the X-axis of each cluster. n, the number of genes present in a cluster