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RT-qPCR of a subset of potential heme transport genes in Aag2 cells in response to heme exposure or deficiency. Real time quantitative PCR of 5 genes differentially expressed in midguts treated with 10 μM heme when compared to 0 μM heme control were selected to validate computational results; AAEL000434, AAEL002406, AAEL002557, AAEL009531 and AAEL012440. Teal bars indicate RT-qPCR relative quantification values and blue bars indicate computational fold change values
