Fig. 5.

dsRNA knockdown of candidate heme importers in Aedes aegypti cultured cells. a RNAi knockdown of heme transporter candidate genes in Aag2 cells. b Gene targets: AAEL000417, AAEL000461, AAEL001495, AAEL002557, AAEL003619, AAEL004513, AAEL008664, AAEL012226, & AAEL012440. Each point on the graph was the average ZnMP fluorescence per cell normalized to the EGFP control for a knockdown replicate. The colors of each point represent the specific experimental day the point was measured. c RNAi with new dsRNA targeting new regions of genes found to effect ZnMP uptake in (b). d RT-qPCR confirmation of RNAi knockdown of DE genes found in cultured cells treated with heme overload or deficiency. e RT-qPCR confirmation of genes knocked down in (d). * Indicates genes that exhibited significantly different fluorescence/RQ than control with p-values below 0.05, ** p-values < 0.01 and *** p-values < 0.001