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. 2020 Aug 31;21:604. doi: 10.1186/s12864-020-06981-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — dsRNA knockdown in adult Aedes aegypti female midguts of candidate genes. a RNAi knockdown of the Ae. aegypti genes AAEL000417, AAEL000461, AAEL001938, AAEL002557, AAEL003318, AAEL004513, AAEL009531 and AAEL012440 in Ae. aegypti female midguts. b ZnMP fluorescence observed after RNAi knockdown of candidate heme transporter genes. Each colored point represents the average ZnMP fluorescence across a midgut normalized to an EGFP control. The colors of each point represent the specific experimental day that the point was observed; each experiment consisted of a sample size of ~n = 30 for each gene. ** Indicates genes that exhibited significantly different fluorescence than control with p-values below 0.01 and *** with p-values < 0.001. c ZnMP fluorescence of simultaneous RNAi knockdown of 4 genes, AAEL000417, AAEL003318, AAEL004513 and AAEL012440 in adult female mosquitoes. d AAEL003318 & AAEL012440 showed RT-qPCR confirmation of RNAi knockdown as they were distinct from the expression in cells targeted with dsEGFP (*** = p-val < 0.001). (e&f) Reproductive potential was measured through area measurements of ovaries at (e) 12 h and (f) 24 h post blood feeding. A slight decrease was observed at 12 h in the mix group (p-val = 0.0191) while no difference was observed at 24 h (p-val = 0.2723)