Table 1.
Ae. aegypti cultured cell heme exposure experimental conditions and differential expression/cluster analysis results
| Cell Type | Incubation Time | Type of Growth Media for Experimental Incubation Time | Differential Expression Comparisons [genes (genes w/TM domains)] |
Cluster Analysis [genes (genes w/TM domains)] |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Normal | Heme-Deficient | Heme-Overexposure | Normal vs Normal + 20 μM Heme | Normal vs 0 μM Heme | Normal vs 10 μM heme | 0 μM vs 10 μM heme | Import-like clusters | Export-like clusters | ||
| Aag2 | 48 h | Schneider’s Drosophila + FBS | N/A | Schneider’s Drosophila + FBS + 20 μM Heme | 551 (211) | 1611(455) | 1391(385) | |||
| Aag2 | 72 h | Schneider’s Drosophila + FBS | Schneider’s Drosophila | Schneider’s Drosophila + 10 μM Heme | 587 (251) | 1990 (737) | 2274 (856) | 745(238) | 687(240) | |
| Aag2 | 72 h | Leibovitz’s L-15 + FBS | Leibovitz’s L-15 | Leibovitz’s L-15 + 10 μM Heme | 4166 (1218) | 4979 (1568) | 3200 (1121) | 905(229) | 670(197) | |
| A20 | 72 h | Leibovitz’s L-15 + FBS | SF900 II | SF900 II + 10 μM Heme | 745 (300) | 2815 (658) | 3692 (942) | 1109(448) | 757(143) | |