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. 2020 Aug 31;20:401. doi: 10.1186/s12870-020-02619-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Confirmation of C2H2 ZFPs specifically expressed in M. truncatula symbiotic nodules. The qRT-PCR result of six selected C2H2 genes. Shown are means ± standard deviations for three biological replicates and three technical replicates of each biological replicate. The relative expression levels of tested genes were normalized by the geometric mean of three endogenous control genes. ‘N.A.’ indicated undetectable expression. The lowercase letters above the bar indicate significant differences (P < 0.05) using ANOVA and Tukey’s test among samples