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. 2020 Aug 10;21(16):5720. doi: 10.3390/ijms21165720

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of PADI2 knockout in the protein and mRNA expression of focal adhesion kinase (FAK), paxillin, and PAK1 in the U937 cells: (A) comparison of the relative protein expression levels of FAK and phospho-FAK in the PADI2 knockout group and the control group; (B) comparison of the relative protein expression levels of paxillin and phospho-paxillin in the PADI2 knockout group and the control group; (C) comparison of the relative protein expression levels of PAK1 in the PADI2 knockout group and the control group; and (D) Representative images showing the relative protein expression levels of FAK, paxillin, and PAK1 in the PADI2 knockout group and the control group; (E) the relative mRNA expression levels of FAK, paxillin, and PAK1 in the U937 cells. In the U937 cells, the protein expression levels of the cell adhesion-related proteins, including FAK, phospho-FAK, paxillin, phospho-paxillin, and PAK1, were performed by Western blotting in the PADI2 knockout group and the control group. The relative protein expression was defined as the ratio of specific protein/hsp90 band intensity.