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. 2020 Aug 9;21(16):5713. doi: 10.3390/ijms21165713

Figure 1.

Figure 1

Expression of peptidylarginine deiminases (PADIs) and citrullinated proteins targeted by anti-citrullinated protein antibodies (ACPAs) in lymph node (LN) tissue and cultured lymph node stromal cells (LNSCs). (A) LN tissue was stained for PADI2 and PADI4 proteins and citrullinated proteins targeted by ACPAs using human monoclonal antibodies C03 and B09. Staining with isotype control (iso) (healthy LN tissues for PADI2 and PADI4 are represented) or control antibody E02 (rheumatoid arthritis (RA) ACPA+ patients LN tissue is represented) was negative. A representative picture of each donor group of the immunohistochemical analysis in LN tissue is displayed (n = 3 per donor group; healthy individuals, RA-risk ACPA− individuals, RA-risk APCA+ individuals, RA ACPA− patients, and RA ACPA+ patients). (B) Cultured LNSCs were stained against PADI2 and PADI4 protein and citrullinated proteins targeted by ACPAs using human monoclonal antibodies C03 and B09. Staining with isotype controls (iso; RA-risk ACPA+ individuals LNSCs for PADI2 and healthy individuals LNSCs for PADI4 are represented) and control antibody E02 (RA ACPA+ patients LNSCs is represented) was negative. A representative picture of each donor per group of the immunohistochemical analysis in cultured LNSCs is displayed (n = 3 per donor group; healthy individuals, RA-risk ACPA− individuals, RA-risk APCA+ individuals, RA ACPA− patients and RA ACPA+ patients, passages 3–11). The larger bar in the left corner of images represents 200 µm and the smaller bar inside the magnified images in (A) represents 20 µm.