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. 2020 Aug 9;21(16):5713. doi: 10.3390/ijms21165713

Figure 6.

Figure 6

Expression of HLA-DR and co-stimulatory molecules on freshly isolated human LNSCs. (A) Human LNs obtained from kidney transplantation recipients were enzymatically digested, and stained for LNSC subsets based on the expression of CD31 and Podoplanin (PDPN) when gated on live CD45− cells. (B) Scatter plot represents the frequencies of different subsets of human LNSCs (hLNSCs) directly after digestion. (C) Stromal cells were gated as live CD45− cells and expression of co-stimulatory and inhibitory molecules on different subclasses of LNSCs and CD45+CD11c+ dendritic cells (DCs) were assessed using flow cytometry. Histograms show the expression of indicated molecules on different stromal cell subsets and DCs. The fluorescence minus one (FMO) was used as negative control. (D) Scatter plots represent mean fluorescence intensity of HLA-DR, CD80, CD86, CD40, and PD-L1 of each subset of LNSCs when gated on live CD45− cells in comparison to DCs. Data are represented as mean with SEM (n = 3–10 kidney recipients).