Figure 3.

Histone demethylase KDM7A is required for the cell proliferation and apoptosis inhibition in bladder cancer cell lines. (A) The time dependent viability changes of control shRNA and KDM7A shRNA expressing bladder cancer cell lines were measured using EZ-Cytox solution. Bars represent means ± SD of three independent experiments. * p < 0.05 (Student’s t-test) versus the control shRNA (sh-cont) group. (B) Crystal violet staining for colonies from the same number of indicated shRNA-expressing stable cells. The average number of colonies is shown in the right panel. Bars represent means ± SD of three independent experiments. * p < 0.05 (Student’s t-test) versus the sh-cont group. (C) The levels of cell cycle-related proteins were measured from the whole cell extracts of control and KDM7A knock-down cells. (D) The apoptotic proteins were detected in the cisplatin-treated KDM7A knock-down bladder cells. Whole-cell lysates were analyzed with the indicated antibodies.