Figure 3.

Effect of polyphenolics in embryogenic cultures during 28 days of dose-response treatments. The Criollo (A–C) and Hass (D–F) cultures were treated with 1, 10, 100, and 1,000 µm of p-hydroxybenzoic acid (A,D), p-coumaric acid (B,E), and trans-ferulic acid (C,F). The effect of polyphenolics was determined based on the number of newly formed globular embryos per plate. A general linear model (GLM) was applied with default settings to determine the statistical significance between treatments. Different letters (a, b and ab) represent a significant difference among treatments (p < 0.05) marked with dash lines.