Table 1.

The endogenous concentration of phenolic compounds (mg g⁻¹ fresh weight) in embryogenic cultures of Criollo and Hass avocado treated with different concentrations of p-coumaric (1 and 10 µM) and trans-ferulic acid (10 and 100 µM) during 6 and 12 h, and 14 and 28 days. The analysis was carried out with dynamic multiple reaction monitoring (dMRM) study. Replicate values, error standard and statistic values are presented in Table S2. p-hydroxybenzoic acid (PHBA), vanillin (VA), p-coumaric acid (p-CA), trans-ferulic acid (t-FA), SA (Sinapic acid) and trans-cinnamic acid (t-CA). We indicated in bold and italic the values described in the text.

Cultivar Time	Treatment	PHBA	VA	p-CA	Quercetin 3,4’-di-O-glucoside	t-FA	SA	Naringin	t-CA
Criollo 0 h	0 µM Control	0.11 ± 0.01	0.11 ± 0.01	0.42 ± 0.01	0.02 ± 0.01	0.23 ± 0.01	0	0.07 ± 0.01	0.02 ± 0.01
	1 µM p-CA	0.11 ± 0.01	0.11 ± 0.01	0.42 ± 0.01	0.02 ± 0.01	0.23 ± 0.01	0	0.07 ± 0.01	0.02 ± 0.01
	1 µM t-FA	0.11 ± 0.01	0.11 ± 0.01	0.42 ± 0.01	0.02 ± 0.01	0.23 ± 0.01	0	0.07 ± 0.01	0.02 ± 0.01
Hass 0 h	0 µM Control	0.21 ± 0.01	0.03 ± 0.03	0.66 ± 0.02	0.01 ± 0.01	0.09 ± 0.01	0	0	0.04 ± 0.01
	10 µM p-CA	0.21 ± 0.01	0.03 ± 0.03	0.66 ± 0.02	0.01 ± 0.01	0.09 ± 0.01	0	0	0.04 ± 0.01
	100 µM t-FA	0.21 ± 0.01	0.03 ± 0.03	0.66 ± 0.02	0.01 ± 0.01	0.09 ± 0.01	0	0	0.04 ± 0.01
Criollo 6 h	0 µM Control	0.58 ± 0.01 ***	0.22 ± 0.01 ***	0.85 ± 0.01 ***	1.66 ± 0.07 ***	1.12 ± 0.01 ***	0.7 ± 0.01 ***	0.34 ± 0.01 ***	0.06 ± 0.01 **
	1 µM p-CA	0.64 ± 0.05 ***	0.06 ± 0.01 **	0.29 ± 0.01 **	0 *	0.12 ± 0.01 **	0 **	0 **	0.4 ± 0.01 ***
	1 µM t-FA	0.38 ± 0.01 **	0.05 ± 0.01 **	0.18 ± 0.01 **	0.03 ± 0.04 **	0.28 ± 0.01 **	0 **	0 **	0.11 ± 0.01 ***
Hass 6 h	0 µM Control	0.29 ± 0.01 *	0.06 ± 0.01 *	0.62 ± 0.01 *	0.33 ± 0.05 *	1 ± 0.01 **	0.86 ± 0.02 ***	0.06 ± 0.01 *	0.03 ± 0 *
	10 µM p-CA	0.25 ± 0.01 *	0.05 ± 0.001 *	0.7 ± 0.01 **	0.02 ± 0.03 **	0.13 ± 0.01 *	0 *	0 *	0.29 ± 0.01 *
	100 µM t-FA	0.32 ± 0.01 *	0.24 ± 0.01 ***	1.39 ± 0.01 ***	0.62 ± 0.02 ***	10.6 ± 0.22 ***	0.64 ± 0.02 **	0.18 ± 0.02 ***	0.98 ± 0.02 ***
Criollo 12 h	0 µM Control	0.21 ± 0.01 **	0.15 ± 0.01 ***	0.61 ± 0.01 ***	0.62 ± 0.04 ***	0.52 ± 0.01 ***	0.72 ± 0.02 ***	0.05 ± 0.01 ***	0.02 ± 0.01 **
	1 µM p-CA	0.25 ± 0.01 ***	0.06 ± 0.01 **	0.46 ± 0.01 **	0 **	0.26 ± 0.01 **	0 **	0 ***	0.15 ± 0.01 ***
	1 µM t-FA	0.18 ± 0 *	0.04 ± 0.01 *	0.18 ± 0.01 *	0 **	0.12 ± 0.01 *	0 **	0 ***	0.03 ± 0.01 **
Hass 12 h	0 µM Control	0.6 ± 0.01 ***	0.11 ± 0.01 *	0.94 ± 0.02 ***	0.18 ± 0.02 ***	0.86 ± 0.07 ***	0.42 ± 0.01 ***	0.21 ± 0.01 ***	0.12 ± 0.01 **
	10 µM p-CA	0.2 ± 0 *	0.03 ± 0.001 *	0.24 ± 0.01 *	0.04 ± 0.01 **	0.13 ± 0.01 *	0 *	0 *	0.08 ± 0.01 *
	100 µM t-FA	0.4 ± 0 *	0.17 ± 0.01 ***	0.48 ± 0.01 *	0.05 ± 0.04 **	0.39 ± 0.01 *	0 *	0.22 ± 0.01 ***	0.08 ± 0.01 *
Criollo 14 days	0 µM Control	1.12 ± 0.02 ***	0.06 ± 0.001 ***	1.28 ± 0.02 ***	0.3 ± 0.01 ***	0.23 ± 0.01 *	0.03 ± 0.02 ***	0 ***	0.01 ± 0.02 ***
	1 µM p-CA	0.09 ± 0.001 *	0.05 ± 0.01 **	0.39 ± 0.01 *	0 **	0.28 ± 0.02 **	0 **	0 ***	0.01 ± 0.02 ***
	1 µM t-FA	0.12 ± 0.01 **	0.02 ± 0.01 *	1.18 ± 0.08 **	0 **	0.56 ± 0.03 ***	0.02 ± 0.01 *****	0 ***	0.02 ± 0.01 ***
Hass 14 days	0 µM Control	0.09 ± 0.01 *	0.03 ± 0.01 ***	0.69 ± 0.03 ***	0 *	0.4 ± 0.01 **	0.01 ± 0.01 **	0 ***	0.02 ± 0.01 ***
	10 µM p-CA	0.11 ± 0.01 **	0.03 ± 0.01 ***	0.24 ± 0.02 *	0.06 ± 0.01 **	0.55 ± 0.03 **	0.1 ± 0.02 ***	0 ***	0.01 ± 0.01 **
	100 µM t-FA	0 *	0 *	0 *	0 *	7.06 ± 3.63 ***	0 **	0 ***	0 *
Criollo 28 days	0 µM Control	0.62 ± 0.01 *	0.05 ± 0.01 **	0.96 ± 0 ***	0 ***	0.72 ± 0.01 ***	0.23 ± 0.01 **	0 ***	0.5 ± 0 ***
	1 µM p-CA	3.56 ± 0.06 ***	0 *	0.05 ± 0.04 *	0 ***	0 *	0 *	0 ***	0 *
	1 µM t-FA	1.35 ± 0.02 **	0.08 ± 0 ***	0.74 ± 0.01 **	0.02 ± 0.04 ***	0.12 ± 0.10 **	1.55 ± 0.12 ***	0 ***	0.03 ± 0 **
Hass 28 days	0 µM Control	0.4 ± 0 *	0.05 ± 0 **	1.14 ± 0.01 ***	0 ***	0.78 ± 0.01 **	0.04 ± 0.01 ***	0 ***	0.01 ± 0 ***
	10 µM p-CA	1.36 ± 0.03 ***	0 *	0.02 ± 0.02 *	0 ***	7.75 ± 4.43 ***	0 *	0 ***	0 **
	100 µM t-FA	1.12 ± 0.01 **	0.03 ± 0 *	0.16 ± 0.01 *	0 ***	0.08 ± 0 **	0.01 ± 0.01 **	0 ***	0 **

1. A general linear model (GLM) for metabolomics data was applied with default settings to determine. the statistical significance between treatments. 2. Data are mean ± standard deviation reported in mµ g⁻¹ of dried EC. 3. *, **, ***, ***** represent a significant difference among treatments (p < 0.05).