Skip to main content
. 2020 Jun 23;31(8):1729–1745. doi: 10.1681/ASN.2019070703

Figure 1.

Figure 1.

Establishment of a model of progressive glomerulosclerosis in male db/db mice with type 2 diabetes. (A) Schematic representation of surgeries in male BKS db/db. Mice underwent uninephrectomy of the right kidney at 6 weeks of age and were fed a sodium-deficient diet until 20 weeks of age (time point 12). (B) Body weight evolution, expressed as mean±SD (db/db 1K (n=34) and WT 1K (n=23)). (C) Blood glucose evolution, expressed as mean±SD (db/db 1K (n=34) and WT 1K (n=23)). (D) GFR evolution, expressed as mean±SD (db/db 1K (n=34) and WT 1K (n=23)). (E) UACR evolution, expressed as mean±SD (db/db 1K (n=26) and WT 1K (n=18)). (F) Glomerulosclerosis in db/db 1K mice, measured as Picro-Sirius Red0positive area (AU). Magnification, ×40. (G) Glomerular area assessment in db/db 1K mice, measured as the glomerular tuft area (AU) in PAS sections. Magnification, ×40. (H) Podocyte number assessment in WT-1 sections from db/db 1K mice. Podocyte density was calculated as the number of podocytes by glomerular tuft area section. Magnification, ×40. For all histologic analyses (F–H), the average number of glomeruli quantified at each time point is specified in Supplemental Table 2. *P value≤0.05 versus time point−2; #P value≤0.05 versus time point 0; §P value≤0.05 versus WT 1K. Statistical analyses performed are summarized in Supplemental Table 4. AU, arbitrary units.