Figure 7.

Three-dimensional reconstruction of glomeruli stained for nephrin upon optical tissue clearing. Images show representative glomeruli of mice from each group. Signal represents nephrin protein within the slit diaphragm. Z-series stacks were obtained from 80-μm kidney slices. Images were collected at 1-μm intervals. Db/db 1K mice treated with MRE show residual defects in coverage representing denudated areas (black areas) with podocyte loss (marked with an asterisk). Arrowheads indicate filtration slits between podocytes showing foot process effacement. Dual MRE+BIO treatment improves these residual defects. Bars, 20 µm. Magnification, ×40.