Figure 2.

Perfusion with linagliptin at human C_max equivalent enhances the contractile recovery of the hearts of aged, diabetic high fat diet-fed (DM-HFD) mice after ischemia reperfusion injury. Percentage recovery of left ventricular developed pressure 40 min after reperfusion (%LVDP R40) in the hearts of male (purple circles) and female (red circles) C57BL/6 mice fed a high fat diet for 12 weeks, before receiving an intraperitoneal injection of streptozotocin (90 mg/kg) and fed a high fat diet for a further 14 weeks. Hearts were perfused with vehicle (sodium pyruvate (2 mmol/L) supplemented Krebs Henseleit buffer, KHB; n = 15) or sodium pyruvate supplemented KHB with 9 nmol/L linagliptin (n = 18), 817 nmol/L sitagliptin (n = 14), 110 ng/mL alogliptin (n = 17) or 24 ng/mL saxagliptin (n = 15), 47 ng/mL 5-hydroxy saxagliptin (n = 15) or 10 nmol/L GLP-1 (n = 18). Age-matched control (n = 17). Values are mean ± S.D.. *p < 0.05, **p < 0.01 by one-way ANOVA followed by Fisher least significant difference post hoc test.