Schematic representation of conserved motifs and segmental duplication analysis. (a) Left: phylogenetic tree of the MACPF family replotted from Figure 1. Right: conserved motif compositions of plant MACPF proteins, predicted by MEME suite. Each of the 10 conserved motifs are represented by color bars. (b) Poaceae MACPF genes were mapped to the chromosomes of Brachypodium distachyon, Oryza sativa, Zea mays, Sorghum bicolor, Setaria italica, and Oropetium thomaeum. MACPF genes were numbered according to their position on the chromosomes. Segmental duplications of MACPF gene pairs are connected by red lines.