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. 2020 Aug 12;21(16):5790. doi: 10.3390/ijms21165790

Figure 1.

Figure 1

Effects of triclosan (TCS) exposure on the developmental competence of parthenogenetically activated porcine embryos. (A) Representative photographs of the PA blastocysts (white asterisks) that developed at indicated concentration of TCS. Bar = 50 μm. (B) Rate of cleavage of embryos treated with different concentrations of TCS after 48 h of in vitro culture (IVC). (C) Developmental rate of embryos for indicated concentrations of TCS at 30 h, 48 h, 96 h, and 144 h of IVC after activation, respectively. (D) Representative images of blastocysts at different stages: early blastocyst (EB), mid-blastocyst (MB), late blastocyst (LB), and expanded blastocyst (ExB). Bar = 50 μm. (E) Quantification of proportion of each blastocyst stage following treatment with indicated concentration of TCS. (F) Nuclear staining of blastomeres cultured in the presence or absence of TCS. Images show blue 4,6-diamidino-2-phenylindole (DAPI) signal. Bar = 50 μm. (G) Quantification of total number of cells in blastocysts cultured with different concentrations of TCS. Data are from three independent experiments, and values represent mean ± standard error of the mean (SEM; * p < 0.05).