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. 2020 Aug 11;21(16):5742. doi: 10.3390/ijms21165742

Figure 2.

Figure 2

Transport of proteins carrying α-Gal through the intestinal Caco-2 monolayer. (A) Time dependent transcytosis of BSA-α-Gal and BSA, n = 4, and (B) human serum albumin (HSA) carrying α-Gal (HSA-α-Gal) and HSA, n = 4. * p < 0.05, ** p ˂ 0.01 and *** p < 0.001 analyzed by two-way ANOVA with Bonferroni’s post hoc test; (C) fluorescent detection of BSA-α-Gal and BSA in Caco-2 lysates resolved on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gel; (D) immunoblot detection of the α-Gal epitope in lysates from Caco-2. MW = Molecular weight markers and CTRL = untreated Caco-2 cells.