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Immunoblot detection of BSA and BSA-α-Gal using (A) polyclonal anti-BSA antibody and (B) monoclonal anti-α-Gal M86 antibody in the endosomal fraction obtained by differential centrifugation of Caco-2 cell lysates after 4 h of incubation, 2°Ab = control of unspecific binding of secondary antibody, MW = Molecular weight markers, and CTRL = unstimulated Caco-2 cells.
