Table 1.
Oligonucleotides used for verification of bacterial strains.
| Gene Symbol | Forward Primer | Reverse Primer | Size (bp) | Reference |
|---|---|---|---|---|
| bexA | CGTTTGTATGATGTTGATCCAGACT | TGTCCATGTCTTCAAAATGATG | 343 | [100] |
| capB | GCTTACGCTTCTATCTCGGTGAA | ACCATGAGAAAGTGTTAGCG | 370 | [6] |
| capF | GCTACTATCAAGTCCAAATC | CGCAATTATGGAAGAAAGCT | 450 | [102] |
| hifA | ATGAAAAAAACACT(AT)CTTGGTAGC | TTAT(CT)CGTAAGCAATT(GT)GGAAACC | 650 | [103] |
| lpsA | TTGAATATCGTTTAGCAC | GCGTGGCGACAATTAGGC | 994 | [38] |
| hia | CGCGGCTTGGGCTGGGTCATTTCT | TCAGCCGTACCGTCAGCATTCAGTTCA | 766 | [32] |
| omp1 | TGATAAATTCGCGCTGGGTG | GGCAGTGCGGTCAGTAAAAT | 454 | this study |
| omp2 | ATAACAACGAAGGGACTAACG | TCTACACCGAATAATACTGCT | 1000 | [104] |
| hel | ATTGGATCCGAATTCTTAAAAGGAAT | ATTAAATATTGGATCCAGTAAAAACTGAGC | 1047 | [105] |
| ompA | CGTGCCTCTGGTTTATTTGC | GCGATTTCTACACGACGGTC | 581 | forward modified from [81] reverse this study |
| omp6 | ACTTTTGGCGGTTACTCTGT | TGTGCCTAATTTACCAGCAT | 273 | [100] |
Abbreviations: bexA, capsule expression; capB, capsular B antigene; capF, capsular F antigene; hifA, H. influenzae fimbrial gene; lpsA, lipopolysaccharide glycosyl transferase; hia, H. influenzae adhesin; omp, outer membrane protein; hel, H. influenzae outer membrane lipoprotein e(P4); ompA, outer membrane porin A.