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. 2020 Aug 10;21(16):5719. doi: 10.3390/ijms21165719

Table A1.

Order, amount, and formula of reagents for preparing 500 mL of SBF.

Order Reagent Amount
1 NaCl 4.018 g
2 NaHCO3 0.178 g
3 KCl 0.114 g
4 K2HPO4 3H2O 0.116 g
5 MgCl2 6H2O 0.159 g
6 1M HCl 19.5 mL
7 CaCl2 0.146 g
8 Na2SO4 0.036 g
9 Tris 3.059 g
10 1M HCl 1.84 mL