Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 31;18:110. doi: 10.1186/s12915-020-00843-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Mutations in βC1 SUMOylation sites abolish pathogenicity. a Design of βC1 SUMOylation motif mutants. SeqLogos indicates conservation in amino acid sequences between βC1. b In vitro SUMOylation assay using purified MBP-βC1 and its SUMOylation motif mutants. Top blot shows SUMOylation conjugates of βC1 probed with anti-SUMO1 and bottom blot is reprobing with anti-MBP. Black arrow indicates intact MBP-βC1. Black and red triangles represent NbSUMO1 and poly-SUMOylated substrates, respectively. c, d Representative phenotypes of GFP-βC1 and its SUMOylation motif mutants in transgenic N. tabacum. c Plants. d Flowers without petals. e Western blot to quantify GFP-βC1 and different SUMOylation motif mutants in transgenic plants using anti-GFP. The star indicates a non-specific band and the arrow shows GFP-βC1. f IP of GFP-βC1 and its SUMOylation motif mutants showing stability of the proteins during transient overexpression. Protein ladder overlaid and false colour applied. g Co-IP of MBP-βC1 and its SUMO mutants during co-overexpression of either NbSUMO1 or NbSUMO1^ΔGG. Black and red triangles indicate NbSUMO1 poly-SUMOylated proteins and NbSUMO1-βC1 conjugates, respectively. Protein marker sizes in kDa are indicated. Size bars in c and d are 36 cm (1.2 ft) and 0.8 cm respectively. P, Ponceau staining for total proteins showing RUBISCO