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. 2020 Aug 31;18:110. doi: 10.1186/s12915-020-00843-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — SUMOylation stabilizes βC1 protein in plants. a Stability of transiently overexpressed MBP-βC1 and mK18, 24R SUMOylation-deficient double mutant in N. tabacum. Samples were collected 3 DPI. b Accumulation of transiently overexpressed MBP-βC1 and mK18, 24R upon MG132 treatment. c Quantification of protein levels after MG132 treatment. n = 3. d, e Same as b and c but with NEM treatment. f Stability of transiently overexpressed MBP-tagged βC1 and its SUMOylation motif triple mutant. The number of PCR cycles (cyc) in RT-PCR experiments has been mentioned. Tukey’s multiple comparison test with four stars representing P value, P ≤ 0.0001 and three stars P ≤ 0.001. P, Ponceau staining for total proteins showing RUBISCO large subunit. Other information same as in Fig. 1