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. 2020 Aug 31;18:110. doi: 10.1186/s12915-020-00843-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Mutations in SIM abolished infectivity of βC1. a–d Habit, flower, leaf and height of transgenic GFP-βC1 and its SIM mutants in N. tabacum transgenic lines (n = 3). Photographs and measurements (graphs) are presented. e Stability of βC1 and SIM mutants overexpressing N-terminal GFP-tagged proteins in transgenic plants. Numbers denote individual transgenic lines. f Stability of SIM mutants during transient overexpression. IP of N-terminal GFP-tagged βC1 and mSIM2,3,4 mutants derived from transient overexpression in N. tabacum. g Western blot showing protein levels during transient overexpression of MBP-tagged βC1 and SIM mutants (structural and null mutants) in N. tabacum. h Quantification of g, the y-axis represents protein expression in arbitrary number. n = 3. Marker sizes in kDa are indicated. Size bar in a 10 cm, b top-panel 0.8 cm and bottom panel 2 cm. P, Ponceau staining for total proteins showing RUBISCO large subunit