Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 31;18:110. doi: 10.1186/s12915-020-00843-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — SUMO/SIM mutants of βC1 block viral systemic movement. a Southern blot viral replication assay in local leaves of transgenic plants expressing p35S::GFP-βC1 or its SUMOylation motif mutants. Infectious DNA A clones were used for infection. Total genomic DNA was treated with S1 nuclease before blotting. b Viral replication assay using SIM mutants of GFP-βC1. c Southern blot showing viral replication and systemic movement with DNA β and SIM/SUMO mutated βC1 variants incorporated in DNA β in local and systemic leaves of N. benthamiana. d Phenotypes of SyYVCV + DNA β or SIM/SUMO mutated βC1 incorporated in DNA β in N. benthamiana (top) and N. tabacum (bottom). Images were taken at 39 DPI. Size bar 5 cm