Fig. 3.

eA3G-BE can induce efficient C-to-T conversions with minimized bystander activity in rabbit embryos. a The six target-site sequences with CC context. Target sequence (black), PAM region (green), target base (red), and mutant amino acid (underlined). NA, not applicable. b, c Frequencies of single C-to-T conversions using rA1-BE and eA3G-BE at six sites in rabbit embryos. CC context (red triangle) and CCC context (green triangle). Target Cs are counting the PAM as positions 21–23. n = ~ 6 blastocysts. d Representative sequencing chromatograms of edited rabbit blastocyst at three target sites using rA1-BE and eA3G-BE systems. Targeted mutations (red arrows) and bystander mutations (blue arrows). The relevant codon identities at the target site are presented under the DNA sequence