Fig. 3.

MiR-199a-3p and miR-199a-5p promotes migration of EMT in HCEs. a, b HCEs were transfected with miR-199a-3p/miR-199a-5p mimics/mimics NC, inhibitors/inhibitor NC. The expression of miR-199a-3p and miR-199a-5p was detected by qRT-PCR (**p < 0.01, ***p < 0.001). HCEs were transfected with miR-199a-3p mimic, miR-199a-5p mimic and mimic NC. c Cell migration was determined by the wound healing assay (*p < 0.05). d Cell migration was determined by the transwell assay (**p < 0.01). e–i HCEs were transfected with miR-199a-3p/miR-199a-5p mimic, mimic NC, miR-199a-3p/miR-199a-5p inhibitor and inhibitor NC. The EMT markers (E-cadherin (CDH1), N-cadherin (CDH2) and Vimentin (VIM)) were detected by qRT-PCR, western blot and immunofluorescence (*p < 0.05, **p < 0.01, ***p < 0.001). j After HCEs were transfected with miR-199a-3p mimic, mimic NC, miR-199a-3p inhibitor and inhibitor NC, COL1A1 and COL3A1 were upregulated only by miR-199a-3p, detected by qRT-PCR (*p < 0.05, ***p < 0.001). k After HCEs were transfected with miR-199a-5p mimic, mimic NC, miR-199a-5p inhibitor and inhibitor NC, MMP1 and COL3A1 were upregulated only by miR-199a-5p, detected by qRT-PCR (*p < 0.05, **p < 0.01, ***p < 0.001). l HCEs were transfected with miR-199a-3p/miR-199a-5p mimics/mimics NC, inhibitors/inhibitor NC. Transfection of the miR-199a-3p mimic and miR-199a-5p mimic decreased the apoptosis cells, while miR-199a-3p inhibitor and miR-199a-5p inhibitor increased the apoptosis cells (*p < 0.05, **p < 0.01)