FIGURE 5.

Histone H4 causes influx of calcium from extracellular medium. Intracellular free calcium changes on exposure to 40 μg/ml histone H4 in the presence (A) or absence (B) of extracellular Ca²⁺ were measured by detecting the fluorescence of Fura‐2 AM loaded neutrophils. fMLP (positive control) or histone H4 was added to the cells at 135 s. Fold changes of area under the curve (AUC) are shown. Results are mean ± sem for 4 experiments. Neutrophil depolarization was significant (P < 0.01) for H4 as compared to cells maintained in PBS alone