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Histone H4 causes sustained neutrophil membrane depolarization. Neutrophils were pre‐incubated with 50 mM Di‐OC5 5 min followed by addition of 40 μg/ml of H4 at time zero. Results are mean ± sem for 4 experiments. Neutrophil depolarization was significant (P < 0.01) for H4 as compared to cells maintained in PBS alone.
