Figure 1.

Schematic representation of the two-step immunomagnetic microbead-based cell separation protocol. Total dermal cells obtained from the processing of healthy human skin samples were subjected to magnetic-activated cell sorting separation. The first step was carried out in order to isolate CD31+ cells (i.e., putative endothelial cells), while the second step, performed on the pool of the remaining CD31− cells, allowed the further separation of CD31−/CD34+ cells (i.e., putative telocytes) from CD31−/CD34− cells (i.e., putative fibroblasts).