Table 1.
Gene | Primers 5′ → 3′ |
---|---|
Decorin (DCN) | F: CTGCTTGCACAAGTTTCCTG R: GACCACTCGAAGATGGCATT |
Vimentin (VIM) | F: CCTCCTACCGCAGGATGTT R: CTGTAGGTGCGGGTGGAC |
a-smooth muscle actin (αSMA) | F: CTGAGCGTGGCTATTCCTTC R: TTCTCAAGGGAGGATGAGGA |
Aldehyde dehydrogenase 3 (ALDH3A1) | F: CCCCTTCAACCTCACCATCC R: GTTCTCACTCAGCTCCGAGG |
Lumican (LUM) | F: CCTGGTTGAGCTGGATCTGT R: TAGGATGGCCCCAGGA |
GAPDH (housekeeping gene) | F: AGCCGAGCCACATCGCTGAG R: TGACCAGGCGCCCAATACGAC |
Hepatocyte growth factor (HGF) | F: GTGAATACTGCAGACCAATGT R: CCAGAGGCATTGTTTTCTTGC |
Insulin-like growth factor-1 (IGF-1) | F: GCTGGTGGATGCTCTTCAGT R: TTGAGGGGTGGGCAATACAT |
Hypoxia-inducible factor-1A (HIF1A) | F: CCAGAAGAACTTTTAGGCCGC R: TGTCCTGTGGTGACTTGTCC |
Vascular endothelial growth factor (VEGF) | F: AGGAGGGCAGAATCATCACG R: CCAGGGTCTCGATTGGATGG |
Reduced expression 1 (REX1) | F: CCTCATTCATGGTCCCCGAG R: CACCCTTCAAAAGTACACCG |
Tumour necrosis factor-inducible gene 6 protein (TSG-6) | F: AAGGATGGGGATTCAAGGAT R: TTTTTCTGGCTGCCTCTAGC |