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. 2020 Aug 14;21(16):5849. doi: 10.3390/ijms21165849

Table 1.

Description of primers used in real-time PCR for corneal stromal keratocytes and adipose-derived stem cells gene expression analysis.

Gene Primers 5′ → 3′
Decorin (DCN) F: CTGCTTGCACAAGTTTCCTG
R: GACCACTCGAAGATGGCATT
Vimentin (VIM) F: CCTCCTACCGCAGGATGTT
R: CTGTAGGTGCGGGTGGAC
a-smooth muscle actin (αSMA) F: CTGAGCGTGGCTATTCCTTC
R: TTCTCAAGGGAGGATGAGGA
Aldehyde dehydrogenase 3 (ALDH3A1) F: CCCCTTCAACCTCACCATCC
R: GTTCTCACTCAGCTCCGAGG
Lumican (LUM) F: CCTGGTTGAGCTGGATCTGT
R: TAGGATGGCCCCAGGA
GAPDH (housekeeping gene) F: AGCCGAGCCACATCGCTGAG
R: TGACCAGGCGCCCAATACGAC
Hepatocyte growth factor (HGF) F: GTGAATACTGCAGACCAATGT
R: CCAGAGGCATTGTTTTCTTGC
Insulin-like growth factor-1 (IGF-1) F: GCTGGTGGATGCTCTTCAGT
R: TTGAGGGGTGGGCAATACAT
Hypoxia-inducible factor-1A (HIF1A) F: CCAGAAGAACTTTTAGGCCGC
R: TGTCCTGTGGTGACTTGTCC
Vascular endothelial growth factor (VEGF) F: AGGAGGGCAGAATCATCACG
R: CCAGGGTCTCGATTGGATGG
Reduced expression 1 (REX1) F: CCTCATTCATGGTCCCCGAG
R: CACCCTTCAAAAGTACACCG
Tumour necrosis factor-inducible gene 6 protein (TSG-6) F: AAGGATGGGGATTCAAGGAT
R: TTTTTCTGGCTGCCTCTAGC
HHS Vulnerability Disclosure