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. 2020 Aug 13;21(16):5818. doi: 10.3390/ijms21165818

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FLIM images of giant unilamellar vesicles (GUVs) formed with DPPC with (+LPS) or without (−LPS) L. dumoffii LPS. The vesicles were exposed (+apoLp-III) or not exposed (−apoLp-III) to FITC-apoLp-III. The upper panel shows the fluorescence emission recorded in the channel representing Rhodamine B (RhB) present in the lipid phase and the middle panel shows fluorescence emission recorded in the channel representing FITC (Figure S2 for a specification of the fluorescence emission channels). The lower panel shows superposition of fluorescence signals recorded in both channels.