Table 2.
Effect of balance between cytokinin and auxin (irrespective of type) on the morphogenetic response in L. spectabilis.
| PGRs Concentration | Total Callus (%) | Callus FW (mg) | Callus DW (%) | Non-Embryogenic Callus (%) | Embryogenic Callus (%) | No. of Embryos Per Explant | |
|---|---|---|---|---|---|---|---|
| Cytokinin | Auxin | ||||||
| low | low | 77.8 a | 242.2 ± 37.3 a | 13.4 a | 41.7 ab | 36.1 b | 3.0 ± 0.7 ab |
| low | high | 90.7 a | 260.5 ± 34.1 a | 13.3 a | 31.5 b | 59.3 a | 4.8 ± 0.8 a |
| high | low | 85.2 a | 245.9 ± 38.0 a | 13.7 a | 56.5 a | 28.7 b | 2.2 ± 0.7 b |
| high | high | 88.9 a | 236.1 ± 36.7 a | 13.4 a | 48.8 ab | 40.0 b | 2.5 ± 0.5 ab |
Means ± standard errors in columns followed by the same letter do not differ significantly according to the Newman-Keuls test at p ≤ 0.05. Low concentration of cytokinin/auxin: 0.5 mg·L−1 BA, NAA, 2,4-D or PIC, 1.0 mg·L−1 IAA. High concentration of cytokinin/auxin: 1.0 mg·L−1 BA, NAA, 2,4-D or PIC, 2.0 mg·L−1 IAA. 2,4-D, 2,4-dichlorphenoxyacetic acid; BA, 6-benzyladenine; DW, dry weight; FW, fresh weight; IAA, indole-3-acetic acid; NAA, 1-naphthaleneacetic acid; PGR, plant growth regulator; PIC, picloram.