Table 3.
Effect of medium composition and explant type on the share of bleeding-heart explants forming embryogenic callus and a mean number of somatic embryos per inoculated explant.
| Medium Symbol | Plant Growth Regulator (mg·L−1) | Explant Type | Mean | ||||
|---|---|---|---|---|---|---|---|
| BA | Auxin | Leaf | Petiole | Internode | |||
| Embryogenic Callus (%) | |||||||
| M1 | 0.5 | IAA | 1.0 | 11.1 bc | 22.2 a–c | 0.0 c | 11.1 DE |
| M2 | 0.5 | 2.0 | 44.4 a–c | 33.3 a–c | 0.0 c | 25.9 CD | |
| M3 | 1.0 | 1.0 | 22.2 a–c | 0.0 c | 0.0 c | 7.4 DE | |
| M4 | 1.0 | 2.0 | 11.1 bc | 33.3 a–c | 0.0 c | 14.8 DE | |
| M5 | 0.5 | NAA | 0.5 | 0.0 c | 0.0 c | 0.0 c | 0.0 E |
| M6 | 0.5 | 1.0 | 55.6 a–c | 44.5 a–c | 22.2 a–c | 40.7 B–D | |
| M7 | 1.0 | 0.5 | 11.1 bc | 0.0 c | 0.0 c | 3.7 DE | |
| M8 | 1.0 | 1.0 | 69.5 a–c | 22.2 a–c | 0.0 c | 30.6 CD | |
| M9 | 0.5 | 2,4-D | 0.5 | 100 a | 55.6 a–c | 33.3 a–c | 63.0 A–C |
| M10 | 0.5 | 1.0 | 88.9 ab | 88.9 ab | 55.6 a–c | 77.8 AB | |
| M11 | 1.0 | 0.5 | 55.6 a–c | 55.6 a–c | 11.1 bc | 40.7 B–D | |
| M12 | 1.0 | 1.0 | 88.9 ab | 22.2 a–c | 11.1 bc | 40.7 B–D | |
| M13 | 0.5 | PIC | 0.5 | 77.8 a–c | 66.7 a–c | 66.7 a–c | 70.4 AB |
| M14 | 0.5 | 1.0 | 100 a | 100 a | 77.8 a–c | 92.6 A | |
| M15 | 1.0 | 0.5 | 55.5 a–c | 88.9 ab | 44.4 a–c | 63.0 A–C | |
| M16 | 1.0 | 1.0 | 77.8 a–c | 88.9 ab | 55.6 a–c | 74.1 AB | |
| Mean | 54.3 A | 45.1 B | 23.6 C | ||||
| No. of embryos per explant | |||||||
| M1 | 0.5 | IAA | 1.0 | 0.3 ± 0.3 cd | 0.8 ± 0.4 b–d | 0.0 d | 0.4 E |
| M2 | 0.5 | 2.0 | 1.6 ± 0.3 b–d | 1.1 ± 0.7 b–d | 0.0 d | 0.9 E | |
| M3 | 1.0 | 1.0 | 1.8 ± 1.0 b–d | 0.0 d | 0.0 d | 0.6 E | |
| M4 | 1.0 | 2.0 | 0.4 ± 0.4 cd | 0.4 ± 0.1 cd | 0.0 d | 0.3 E | |
| M5 | 0.5 | NAA | 0.5 | 0.0 d | 0.0 d | 0.0 d | 0.0 E |
| M6 | 0.5 | 1.0 | 2.0 ± 0.5 b–d | 1.9 ± 1.0 b–d | 0.3 ± 0.2 cd | 1.4 DE | |
| M7 | 1.0 | 0.5 | 0.1 ± 0.1 d | 0.0 d | 0.0 d | 0.0 E | |
| M8 | 1.0 | 1.0 | 2.2 ± 0.2 b–d | 0.3 ± 0.3 cd | 0.0 d | 0.9 E | |
| M9 | 0.5 | 2,4-D | 0.5 | 9.2 ± 2.7 a–d | 7.1 ± 3.6 a–d | 2.3 ± 0.2 b–d | 6.2 A–C |
| M10 | 0.5 | 1.0 | 8.9 ± 1.3 a–d | 9.1 ± 3.1 a–d | 3.9 ± 1.8 a–d | 7.3 AB | |
| M11 | 1.0 | 0.5 | 5.1 ± 4.2 a–d | 4.3 ± 3.4 a–d | 0.1 ± 0.1 d | 3.2 C–E | |
| M12 | 1.0 | 1.0 | 5.6 ± 0.3 a–d | 2.1 ± 2.1 b–d | 1.3 ± 1.3 b–d | 3.0 C–E | |
| M13 | 0.5 | PIC | 0.5 | 8.8 ± 3.5 a–d | 4.7 ± 1.9 a–d | 2.8 ± 0.9 b–d | 5.4 BC |
| M14 | 0.5 | 1.0 | 9.7 ± 1.3 ab | 11.4 ± 0.5 a | 7.2 ± 2.1 a–d | 9.4 A | |
| M15 | 1.0 | 0.5 | 2.9 ± 2.2 b–d | 9.3 ± 3.9 a–c | 2.2 ± 0.6 b–d | 4.8 B–D | |
| M16 | 1.0 | 1.0 | 5.9 ± 1.1 a–d | 6.3 ± 1.7 a–d | 5.9 ± 3.4 a–d | 6.0 A–C | |
| Mean | 4.0 A | 3.7 A | 1.6 B | ||||
Means ± standard errors in rows and columns followed by the same letter do not differ significantly according to the Newman-Keuls test at p ≤ 0.05. Upper-case letters refer to the main effects (irrespectively). Lower-case letters refer to the interaction between the two studied independent variables. 2,4-D, 2,4-dichlorphenoxyacetic acid; BA, 6-benzyladenine; IAA, indole-3-acetic acid; M, medium; NAA, 1-naphthaleneacetic acid; PIC, picloram.