Figure 3.

The knockdown of CAIX reduces hypoxia-induced GBM motility and monocyte adhesion to GBM. (A) U87 and U251 cells were transfected with empty vector or wild-type CAIX for 24 h and exposed to hypoxic conditions (1% O₂) for 24 h. CAIX expressions were determined by Western blot using whole cell lysates. The pH values of the culture media are shown in (B). In vitro migration activities were measured after 24 h with a transwell assay and then visualized using a digital camera (C, left panel). The quantification of GBM migration abilities is shown in the right panel. BCECF-AM-labeled-THP-1 was added to GBM cells for 30 min, and, subsequently, the adherence of THP-1 was measured by fluorescence microscopy (D, left panel). The monocyte adhesion abilities are shown in right panel. * p < 0.05 compared with the empty vector group. # p < 0.05 compared with the shCAIX group. (Student’s t-test). Quantitative data are presented as the mean ± standard error (representative of n = 3).